Active Recombinant Mouse F3 protein, His-tagged

• Cat.No.: F3-426M
• Product Overview: Recombinant Mouse F3 protein(NP_034301.3)(Met1-Glu251), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 1-251 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is < 5 μg/ml.
• Molecular Mass: The secreted recombinant mouse F3 consists of 234 amino acids and has a calculated molecular mass of 26.8 kDa. As a result of glycosylation, the apparent molecular mass of the recombinant protein is approximately 40-45 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Met1-Glu251
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 98 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: F3 coagulation factor III [ Mus musculus ]
• Official Symbol: F3
• Synonyms: F3; coagulation factor III; tissue factor; TF; Cf3; Cf-3; CD142; AA409063
• Gene ID: 14066
• mRNA Refseq: NM_010171
• Protein Refseq: NP_034301