Active Recombinant Mouse F3 protein, His-tagged, Low Endotoxin

• Cat.No.: F3-426M
• Product Overview: Recombinant Mouse F3 protein(P20352)(29-251 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 29-251 aa
• Form: Lyophilized from 0.22 μm filtered solution in 50mM Tris, 150mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <5 µg/mL.
• Molecular Mass: The protein has a predicted MW of 27 kDa. Due to glycosylation, the protein migrates to 42-55 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: Store at -20 to -80°C for 12 months as supplied from date of receipt.-80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: F3 coagulation factor III [ Mus musculus ]
• Official Symbol: F3
• Synonyms: F3; coagulation factor III; tissue factor; TF; Cf3; Cf-3; CD142; AA409063
• Gene ID: 14066
• mRNA Refseq: NM_010171
• Protein Refseq: NP_034301