Active Recombinant Mouse Fcgr4 protein, His-Avi-tagged

• Cat.No.: Fcgr4-3996M
• Product Overview: Recombinant Mouse Fcgr4 protein(NP_653142.2)(Met1-Gln203), fused with His-Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: Met1-Gln203
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized mouse CD16-2-AVI-His at 10 μg/ml (100 μl/well) can bind recombinant human IgG1 (Fc). The EC50 of human IgG1 (Fc) is 0.34-0.78 μg/ml.
• Molecular Mass: The recombinant mouse FCGR4 consists of 214 amino acids and has a predicted molecular mass of 24.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmFCGR4 is approximately 25-35 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Fcgr4 Fc receptor, IgG, low affinity IV [ Mus musculus ]
• Official Symbol: Fcgr4
• Synonyms: FCGR4; Fc receptor, IgG, low affinity IV; Fc gammaRIV; Fc receptor-like 3; Fc fragment of IgG, low affinity IIIa, receptor for (CD16); Fc fragment of IgG, low affinity III, receptor for (CD16); Fcrl3; CD16-2; FcgRIV; Fcgr3a; FcgammaRIV; 4833442P21Rik
• Gene ID: 246256
• mRNA Refseq: NM_144559
• Protein Refseq: NP_653142