Active Recombinant Mouse Fibroblast Growth Factor Receptor 2, Fc-tagged

• Cat.No.: Fgfr2-551M
• Product Overview: Recombinant Mouse Fgfr2protein was expressed in Murine myeloma cell line with the Fc part of humanIgG1 Pro100-Lys330 (C-terminus) and Arg22-Glu378 & Asn314His (N-terminus).

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 22-378 a.a.
• Description: Fibroblast growthfactor receptor 2 alpha (Fgfr2α) is a 140 kDa type I transmembrane receptortyrosine kinase that mediates the heparan sulfate proteoglycandependentbiological activity of several FGFs. It is a member of the FGFR family ofproteins whose extracellular domains contain three immunoglobulin-Ig-likedomains with a series of acidic residues between the first and second Ig-likedomains. Multiple forms of Fgfr2 are generated by alternative splicing. Alphaisoforms contain all three Ig-like domains, while beta forms lack the firstIg-like domain.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Arg22, Ser24, andLeu27
• Molecular Weight: 66.2 kDa
• Activity: Measuredby its binding ability in a functional ELISA. 200 ng/mL of biotinylatedrmFGF1 is mixed with serially diluted rmFgfr2α (IIIb)/Fc. Followingincubation, the gfr2α (IIIb)/Fc FGF1/ biotin complex is captured on aStreptavidin coated plate. Bound Fgfr2α (IIIb)/Fc is measured using HRPlabeled Gt x Human IgG Fc. The concentration of rmFgfr2α (IIIb)/Fc thatproduces 50% of the optimal binding was found to be approximately 150-600ng/mL.
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 105-120kDa, reducing conditions
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 100 μg/mL in sterile PBS
• Storage: Use a manualdefrost freezer and avoid repeated freezethaw cycles. 12 months from date of receipt,-20 to -70°C as supplied. 1 month, 2 to 8 °C under sterile conditions after reconstitution.3 months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Fgfr2 fibroblast growth factorreceptor 2 [ Mus musculus ]
• Official Symbol: Fgfr2
• Synonyms: Fgfr2;fibroblast growth factor receptor 2; Bek; svs; KGFR; Fgfr7; Fgfr-2; Fgfr-7;KGFRTr; AU043015; AW556123; fibroblast growth factor receptor 2; keratinocytegrowth factor receptor; EC 2.7.10.1; CD332; Ect1
• Gene ID: 14183
• mRNA Refseq: NM_010207
• Protein Refseq: NP_034337
• UniProt ID: P21803
• Chromosome Location: 7 F3; 7
• Pathway: Downstream signalingof activated FGFR; ESC Pluripotency Pathways; FGFR ligand binding andactivation; FGFR2 ligand binding and activation; IRS-mediated signalling;IRS-related events; Insulin receptor signalling cascade; MAPK signalingpathway; PI-3K cascade; Pathways in cancer; Phospholipase C-mediated cascade;Regulation of Actin Cytoskeleton; Endocytosis; Signaling by FGFR; Signalingby Insulin receptor
• Function: ATP binding;fibroblast growth factor 1 binding; fibroblast growth factor binding;fibroblast growth factor-activated receptor activity; heparin binding; kinaseactivity; nucleotide binding; protein binding; protein homodimerizationactivity; protein kinase activity; protein tyrosine kinase activity; receptoractivity; transferase activity; transmembrane receptor protein tyrosinekinase activity