Active Recombinant Mouse Figf protein, hFc-tagged

• Cat.No.: Figf-870M
• Product Overview: Recombinant Mouse Figf protein(P97946)(Phe98-Ser206), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 98-206 a.a.
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: 1. Measured by its binding ability in a functional ELISA.2. Immobilized human VEGFR3-His at 10 μg/mL (100 μl/well) can bind mouse Fc-VEGFD, The EC50 of mouse Fc-VEGFD is 49 ng/mL.3. Immobilized mouse VEGFR3-His at 10 μg/mL (100 μl/well) can bind mouse Fc-VEGFD, The EC50 of mouse Fc-VEGFD is 44 ng/mL.
• Molecular Mass: The recombinant mouse FIGF/Fc is a disulfide-linked homodimer. The reduced monomer comprises 369 amino acids and has a calculated molecular mass of 40.6 kDa.The apparent molecular mass of the protein is approximately 45 and 36 kDa in SDS-PAGE under reducing conditions.
• Proteinlength: Phe98-Ser206
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Figf c-fos induced growth factor [ Mus musculus ]
• Official Symbol: Figf
• Synonyms: FIGF; c-fos induced growth factor; vascular endothelial growth factor D; c-fos-induced growth factor; Vegfd; VEGF-D; AI325264
• Gene ID: 14205
• mRNA Refseq: NM_010216
• Protein Refseq: NP_034346