Active Recombinant Mouse Glycoprotein 5 (platelet), His-tagged

• Cat.No.: Gp5-5621M
• Product Overview: Recombinant Mouse Gp5protein was expressed in Murine myeloma cell line with a C-terminal 6-Histag. Met1-Gly522.

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: His
• Protein Length: 1-522 a.a.
• Description: Gp5 is an 83 kDatype I transmembrane (TM) glycoprotein of the leucine-rich repeat (LRR)family. It is expressed exclusively within the platelet / megakaryocytelineage, where it noncovalently interacts with other platelet TM LRRproteins, GPIbα/β and GPIX, at a ratio of one GPV to two of each othersubunit. The GPI-V-IX complex tethers platelets to von Willebrand factor onthe surface of injured endothelial cells. Absence of the complex results inBernard-Soulier syndrome, a rare bleeding disorder.
• Form: Lyophilized from a0.2 μm filtered solution in PBS.
• N-terminalSequence: Gln17
• Molecular Weight: 57.5 kDa
• Activity: Measuredby its binding ability in a functional ELISA. When Recombinant Mouse Gp5 is coated at 5 μg/mL, the concentration of RecombinantHuman vWFA2 that produces 50% optimal binding response is found to beapproximately 0.25-1.25 μg/mL
• Endotoxin Level: <1.0 EUper 1 μg of the protein by the LAL method.
• SDS-PAGE: 70-90kDa, reducing conditions
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: Reconstituteat 500 μg/mL in PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months fromdate of receipt, -20 to -70°C as supplied. 1 month, 2 to 8 °C under sterileconditions after reconstitution. 3 months, -20 to -70°C under sterileconditions after reconstitution.

Gene Information

• Gene Name: Gp5 glycoprotein 5 (platelet) [Mus musculus ]
• Official Symbol: Gp5
• Synonyms: Gp5;glycoprotein 5 (platelet); GPV; platelet glycoprotein V; GP V; glycoprotein V(platelet); Uncharacterized protein
• Gene ID: 14729
• mRNA Refseq: NM_008148
• Protein Refseq: NP_032174
• UniProt ID: Q9QZU3
• Chromosome Location: 16 B2; 16
• Pathway: ECM-receptorinteraction; Formation of Fibrin Clot (Clotting Cascade); GP1b-IX-Vactivation signalling; Hematopoietic cell lineage; Hemostasis; IntrinsicPathway; Platelet Adhesion to exposed collagen; Platelet Aggregation (PlugFormation); Platelet activation
• Function: collagen binding