| Species : |
Mouse |
| Source : |
Mammalian Cells |
| Tag : |
His |
| Protein Length : |
1-265 a.a. |
| Description : |
Gp6 is a63 kDa platelet/megakaryocyte-specific type I transmembrane glycoprotein ofthe immunoglobulin superfamily that is an important collagen receptor andinitiator of platelet activation, aggregation and thrombus generation. Gp6 isalso a secondary receptor required for platelet spreading on laminin. Mouse Gp6contains a 21 amino acid (aa) signal sequence, a 244 aa extracellular domain (ECD)that has two C-type Ig-like domains followed by a mucin-like, presumablyO-glycosylated Ser-Thr-rich region, a 21 aa transmembrane (TM) domain and a27 aa cytoplasmic tail. Mouse Gp6 ECD shares 70%, 85%, 69% and 66% aaidentity with human, rat, equine and canine Gp6 ECD, respectively. |
| Form : |
Lyophilized from a0.2 um filtered solution in PBS. |
| N-terminalSequence : |
Gly24 |
| MolecularWeight : |
27.8 kDa (monomer) |
| Activity : |
Measuredby its binding ability in a functional ELISA. When cross-linked with 10 μg/mLof Mouse polyHistidine Monoclonal Antibody. Recombinant Mouse Gp6 binds toCollagen I (coated at 10 μg/mL, 100 μL/well) with an apparent KD < 90 nM. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
45-60kDa, reducing conditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 400μg/mL in PBS. |
| Storage : |
Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution. |
