| Species : |
Mouse |
| Source : |
Mammalian Cells |
| Tag : |
His |
| Protein Length : |
26-549 a.a. |
| Description : |
Theglypicans (glypiated proteoglycans) are a small multigene family ofGPI-linked proteoglycans that likely play a key role in embryonicmorphogenesis. There are six known mammalian glypicans. They all share a common-sizedprotein core of 60-70 kDa, an N-terminus which likely forms a compact globulardomain, 14 conserved cysteines that form multiple intrachain disulfide bonds,and a number of C-terminal N-and O-linked carbohydrate attachment sites.Based on exon organization and the location of O-linked glycosylation sites,at least two subfamilies of glypicans are known, with one subfamily containingglypicans-1, 2, 4, and 6, and another subfamily containing glypicans-3 and 5. |
| Form : |
Lyophilized from a0.2 um filtered solution in PBS with BSA as a carrier protein. |
| N-terminalSequence : |
Gly26 |
| MolecularWeight : |
59.1 kDa |
| Activity : |
Measuredby its binding ability in a functional ELISA. Immobilized rm Gpc5 at5μg/mL (100μL/well) can bind rhFGF-basic with a linear range of 0.16-10ng/mL. |
| Endotoxin Level : |
<1.0EU per 1μg of the protein by the LAL method. |
| SDS-PAGE : |
61-65kDa, reducing conditions. |
| Purity : |
>95%,by SDS-PAGE under reducing conditions and visualized by silver stain. |
| Reconstitution : |
Reconstituteat 100μg/mL in sterile PBS containing at least 0.1% human or bovine serumalbumin. |
| Storage : |
Use a manual defrost freezer and avoid repeated freezethawcycles. 12months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °Cunder sterile conditions after reconstitution. 3 months, -20 to -70 °C understerile conditions after reconstitution. |
