Active Recombinant Mouse Hgf protein(Met1-Leu728), His-tagged

Species :	Mouse
Source :	HEK293
Tag :	His
Protein Length :	Met1-Leu728
Form :	Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity :	Measured by its ability to neutralize TGF-beta mediated inhibition on Mv-1-Lu cell prolifeRation. The ED50 for this effect is typically 1-8 ng/mL.
Molecular Mass :	The recombinant mouse HGF comprises 707 amino acids and has a predicted molecular mass of 80.8 kDa. The apparent molecular mass of the protein is approximately 92 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin :	< 1.0 EU per μg of the protein as determined by the LAL method
Purity :	> 70 % as determined by SDS-PAGE
Storage :	Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution :	It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Name	Hgf hepatocyte growth factor [ Mus musculus ]
Official Symbol	Hgf
Synonyms	HGF; hepatocyte growth factor; SF; scatter factor; hepatopoeitin-A; hepatopoietin-A; NK1; NK2; HGF/SF; SF/HGF; C230052L06Rik;
Gene ID	15234
mRNA Refseq	NM_010427
Protein Refseq	NP_034557