Active Recombinant Mouse Histocompatibility 60a, Fc-tagged

• Cat.No.: H60a-5636M
• Product Overview: RecombinantMouse H60a protein was expressed in Murine myeloma cell line with the Fc partof Human IgG1 Pro100-Lys330 (C-terminus) and Mouse Mouse H60a Asp30-Gln212(N-terminus).

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 30-212 a.a.
• Description: H60a wasoriginally described as an immunodominant histocompatibility antigen that isexpressed in BALB mice but not in B6 mice. More recently it was reported to functionas a ligand for mouse NKG2D, an activating receptor found on NK cells, onsome T cell subsets, and on stimulated macrophages. H60a shares approximately25 percent amino acid identity with the Rae-1 family, a small group ofproteins that also function as ligands for mouse NKG2D. H60a and the Rae-1proteins are distantly related to MHC class I proteins, but they possessesonly the α1 and α2 Ig-like domains, and have no capacity to bind peptide orinteract with β2-microglobulin.
• Form: Lyophilizedfrom a 0.2 um filtered solution in PBS.
• N-terminalSequence: Asp30
• MolecularWeight: 47.8 kDa (monomer)
• Activity: Measuredby its ability to bind to immobilized rmNKG2D/Fc Chimera in a functionalELISA.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 75-90kDa, reducing conditions.
• Purity: >95%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: H60a histocompatibility 60a [ Musmusculus ]
• Official Symbol: H60a
• Synonyms: H60a;histocompatibility 60a; H60; histocompatibility 60; Histocompatibilityantigen 60a
• Gene ID: 15101
• mRNA Refseq: NM_010400
• Protein Refseq: NP_034530
• UniProt ID: Q3TDZ7
• Chromosome Location: 10
• Pathway: Natural killer cellmediated cytotoxicity
• Function: natural killer celllectin-like receptor binding