Species : |
Mouse |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
1-460 a.a. |
Form : |
Lyophilized from sterile 20mM MES, 150mM NaCl, pH 6.0Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : |
Measured by its ability to bind protoporphyrin IX (PPPIX). Recombinant mouse Hemopexin binds >15 μM PPPIX, resulting in a 50% decrease in the fluorescence signal of mouse Hemopexin. |
Molecular Mass : |
The recombinant mouse HPX consists of 448 amino acids and has a predicted molecular mass of 50.4 kDa. The apparent molecular mass of the rm HPX is approximately 60-70 kDa in SDS-PAGE under reducing conditions due to glycosylation. |
Proteinlength : |
Met1-Gln460 |
Endotoxin : |
< 1.0 EU per μg of the protein as determined by the LAL method |
Purity : |
> 98 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |