Active Recombinant Mouse Igfbp5 Protein, His&Avi&Flag-tagged, Biotin-labeled

• Cat.No.: Igfbp5-11099M
• Product Overview: Biotinylated Mouse IGFBP-5 Protein, His, Avitag&Flag Tag is expressed from human 293 cells (HEK293). It contains AA Leu 20 - Glu 271 (Accession # Q07079). This protein carries a polyhistidine tag and an Avi tag (Avitag) at the N-terminus followed by a Flag tag at the C-terminus. Predicted N-terminus: His

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&Flag&His
• Protein Length: 20-271 aa
• Conjugation/Label: Biotin
• Description: IGFBP5 stands for Insulin-like Growth Factor Binding Protein 5. It is a protein that belongs to the insulin-like growth factor binding protein (IGFBP) family. IGFBP5 plays a crucial role in regulating the activity and distribution of insulin-like growth factors (IGFs) in the body. Enables insulin-like growth factor I binding activity.
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 0.3 M Arginine with trehalose as protectant.
• Specificity: Protein Ratio: Passed as determined by the HABA assay/binding ELISA.
• Bio-activity: Immobilized Biotinylated Mouse IGFBP-5 Protein, His, Avitag&Flag Tag at 1 μg/mL (100 μL/well) on streptavidin precoated (0.5 μg/well) plate can bind Mouse IGF-I, Fc Tag with a linear range of 1-20 ng/mL.
• Molecular Mass: The protein has a calculated MW of 33.2 kDa. The protein migrates as 35-38 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Purity: >80% as determined by SDS-PAGE.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Igfbp5 insulin-like growth factor binding protein 5 [ Mus musculus ]
• Official Symbol: IGFBP5
• Synonyms: IGFBP5; insulin-like growth factor binding protein 5; insulin-like growth factor-binding protein 5; IBP-5; IGF-binding protein 5; IGFBP-5; AI256729; AW208790; IGFBP-5P
• Gene ID: 16011
• mRNA Refseq: NM_010518
• Protein Refseq: NP_034648
• UniProt ID: Q07079