Active Recombinant Mouse IgG2aFc protein, Avi-tagged, Biotinylated

• Cat.No.: IgG2aFc-0213M
• Product Overview: Biotinylated Recombinant Mouse IgG2aFc protein(Glu98-Lys330), fused with Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi
• Protein Length: 98-330 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Recombinant Protein A at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2a Fc, Avitag with a linear range of 0.8-13 ng/mL.Immobilized Mouse CD16-2, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Mouse IgG2a Fc, Avitag with a linear range of 0.01-0.313 μg/mL.
• Molecular Mass: This protein carries an Avi tag at the C-terminus.The protein has a calculated MW of 28.2 kDa. The protein migrates as 32-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Glu98-Lys330
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.
• Conjugation: Biotin

Gene Information

• Official Symbol: IgG2aFc