Active Recombinant Mouse IgG3Fc protein

• Cat.No.: IgG3Fc-1572M
• Product Overview: Recombinant Mouse IgG3Fc protein(Pro98-Pro327) was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Non
• Protein Length: 98-327 a.a.
• Form: Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5.
• Bio-activity: Immobilized Mouse IgG3 Fc Protein, Tag Free at 5 μg/mL (100 μL/well) can bind Biotinylated Human CD64, His,Avitag with a linear range of 0.6-10 ng/mL.
• Molecular Mass: This protein carries no "tag".The protein has a calculated MW of 26.1 kDa. The protein migrates as 30-35 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Pro98-Pro327
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.>90% as determined by SEC-MALS.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.