Active Recombinant Mouse IGHE protein, His-Avi-tagged, Biotinylated, Low Endotoxin

• Cat.No.: IGHE-3692MB
• Product Overview: Biotinylated Recombinant Mouse IGHE protein(P06336)(198-421 aa), fused with C-terminal His and Avi tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Avi&His
• Protein Length: 198-421 aa
• Conjugation/Label: Biotin
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Fc epsilon RI alpha, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse IgE, His Avi Tag with the EC50 of 42.0ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 28.2 kDa. Due to glycosylation, the protein migrates to 35-45 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Official Symbol: IGHE