Active Recombinant Mouse IL12A&IL12B protein, His-tagged

• Cat.No.: IL12A&IL12B-28M
• Product Overview: This product contains IL12A & IL12B heterodimer. A DNA sequence encoding the p35 subunit of mouse IL12, termed as IL12A (P43431) (Met 1-Ala 215) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 1; A DNA sequence encoding the p40 subunit of mouse IL12, termed as IL12B (P43432) (Met 1-Ser 335) was fused with a polyhistidine tag at the C-terminus, constructed the plasmid 2. The two plasmids were co-expressed and the heterodimer was purified.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met 1-Ala 215&Met 1-Ser 335
• Form: Lyophilized from sterile PBS, pH 7.4
• Bio-activity: 1. Measured by its ability to bind biotinylated recombinant mouse IL12A&IL12BRB2 in a functional ELISA.
  2. Measured by its ability to induce Interferon gamma secretion by human natural killer lymphoma NK-92 cells. The ED50 for this effect is 2-10 ng/mL.
• Molecular Mass: The recombinant mouse IL12A&IL12B heterodimer of IL12A&IL12BA/IL12A&IL12BB, comprises 528 (204 + 324) amino acids and has a calculated molecular mass of 60.3 (23.1 + 37.2) kDa. The apparent molecular mass of rh IL12A&IL12B heterodimer is approximately 28 & 47 kDa respectively in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.