Active Recombinant Mouse Il12b Protein

• Cat.No.: Il12b-1235M
• Product Overview: Purified recombinant protein of Mouse interleukin 12b (Il12b) (Met23-Ser335) without tag was was expressed Sf9 cell.

Specification

• Description: This gene encodes the beta subunit p40 of the Interleukin 12 (IL-12) family of cytokines. Members of the IL-12 family form heterodimers consisting of heavy and light subunits linked by disulfide bonds. The product of this gene, p40, is a subunit of interleukins IL-12 and IL-23.
• Source: Insect Cell
• Species: Mouse
• Bio-activity: The ED50 is 1- 4 ng/ml corresponding to a specific activity of 1.0 - 0.25 x 10^6 units/mg, determined by the dose dependent inhibition of IL-12-dependent IFNγ production.
• Molecular Mass: 35.8 kDa
• Endotoxin: Less than 0.01 ng per µg protein as determined by the LAL method
• Purity: >98%, as determined by Coomassie stained SDS-PAGE.
• Stability: Unopened vial can be stored between 2 centigrade and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 centigrade and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: Protein formulated and vialed at >50 µg/mL in specified buffer
• Storage Buffer: 20 mM Tris-HCl, pH8.0, 0.1 M NaCl
• Protein length: Met23-Ser335

Gene Information

• Gene Name: Il12b interleukin 12b [ Mus musculus (house mouse) ]
• Official Symbol: Il12b
• Synonyms: Il12b; interleukin 12b; p40; Il-12b; Il12p40; Il-12p40; interleukin-12 subunit beta; CLMF p40; IL-12 p40 subunit; IL-12 subunit p40; IL-23 subunit p40; cytotoxic lymphocyte maturation factor 40 kDa subunit; interleukin-12 p40 subunit
• Gene ID: 16160
• mRNA Refseq: NM_001303244
• Protein Refseq: NP_001290173
• UniProt ID: P43432

Reviews

04/08/2020

Good for cytokine assays.

01/06/2020

Effective in Th2 cell differentiation.

01/12/2019

High yield in expression.

Q&As

Could you explain the use of ChIP-seq in studying IL12B transcriptional regulation?

ChIP-seq identifies regulatory elements controlling IL12B gene expression.

Can you elaborate on the use of reporter assays to monitor IL12B promoter activity?

Reporter assays monitor IL12B promoter activity under varying conditions.

What methods evaluate IL12B's response to different pathogenic stimuli in primary cells?

Primary cell stimulation assays gauge IL12B response to pathogens.

How does siRNA knockdown of IL12B elucidate its role in immune cell activation?

siRNA knockdown uncovers IL12B's role in immune cell activation pathways.

How does recombinant protein expression and purification aid in IL12B functional studies?

Recombinant protein expression enables functional IL12B studies in vitro.

How does co-immunoprecipitation validate IL12B's association with signaling partners?

Co-IP validates IL12B's binding to specific signaling partners.

What techniques are employed to analyze the impact of IL12B genetic variants on protein function?

Genetic variant analysis reveals impacts on IL12B protein function.

How does flow cytometry reveal IL12B's expression dynamics in different immune cell subsets?

Flow cytometry profiles IL12B expression across diverse immune cell types.

What experimental approaches determine IL12B's cytokine secretion kinetics upon stimulation?

Cytokine secretion assays measure IL12B's response kinetics to stimuli.

What are the methods to assess IL12B's protein-protein interactions in a cellular context?

Co-IP and immunoprecipitation assess IL12B's interactions with other proteins.