Species : |
Mouse |
Source : |
E.coli |
Tag : |
Non |
Protein Length : |
Ser115-Ser270 |
Form : |
Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
Bio-activity : |
1. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL.2. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells.The ED50 for this effect is typically 15-60 ng/mL. |
Molecular Mass : |
The recombinant mouse IL1A consists of 157 amino acids and migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions as predicted. |
Purity : |
> 92 % as determined by SDS-PAGE |
Storage : |
Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : |
It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |