Active Recombinant Mouse Il1a protein
| Cat.No. : | Il1a-306M |
| Product Overview : | Recombinant Mouse Il1a protein(NP_034684.2)(Ser115-Ser270) was expressed in E. coli. |
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| Source : | E. coli |
| Species : | Mouse |
| Tag : | N/A |
| Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. |
| Bio-activity : | 1. Immobilized mouse IL1A at 10 μg/mL (100 μl/well) can bind mouse IL1R1, The EC50 of mouse IL1R1 is 0.13 μg/mL.2. Measured by its ability to induced Interferon gamma secretion by human natural killer lymphoma NK-92 cells.The ED50 for this effect is typically 15-60 ng/mL. |
| Molecular Mass : | The recombinant mouse IL1A consists of 157 amino acids and migrates as an approximately 18 kDa band in SDS-PAGE under reducing conditions as predicted. |
| Protein Length : | Ser115-Ser270 |
| Purity : | > 92 % as determined by SDS-PAGE |
| Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
| Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
| Gene Name : | Il1a interleukin 1 alpha [ Mus musculus ] |
| Official Symbol : | Il1a |
| Synonyms : | IL1A; interleukin 1 alpha; interleukin-1 alpha; IL-1 alpha; Il-1a; |
| Gene ID : | 16175 |
| mRNA Refseq : | NM_010554 |
| Protein Refseq : | NP_034684 |
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| ◆ Lysates | ||
| IL1A-2911HCL | Recombinant Human IL1A cell lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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Q&As (10)
Ask a questionCross-talk between IL-1α's and other cytokines is dissected through methods such as co-immunoprecipitation, ELISA, and cytokine profiling arrays.
IL-1α's paradoxical roles are dissected using conditional knockout mice, 3D culture systems, and molecular analyses to delineate context-specific effects.
Single-cell RNA sequencing uncovers heterogeneity in IL-1α-responsive cell populations and their roles, revealing distinct molecular profiles and functions.
IL-1α's intricate pro-inflammatory signaling pathways are decoded using advanced techniques like mass spectrometry, phosphoproteomics, and bioinformatics.
Genetic editing tools like CRISPR-Cas9 unveil IL-1α's contributions to diseases by generating knockout models and studying resulting phenotypic changes.
In vitro co-culture systems are manipulated to study IL-1α-mediated responses, elucidating immune-stromal cell interactions via cytokine-specific neutralization.
Specific cellular receptors and effectors influenced by IL-1α are identified through techniques like co-immunoprecipitation, ChIP-seq, and siRNA knockdown.
Live-cell microscopy captures real-time IL-1α release dynamics and its impact on neighboring cells, facilitated by fluorescent tagging and high-resolution imaging.
Longitudinal studies and statistical modeling establish the quantitative relationship between IL-1α levels and disease progression, offering predictive insights.
Multi-omics analyses provide a holistic view of IL-1α signaling's impact, integrating transcriptomics, proteomics, and metabolomics data for comprehensive insights.
Customer Reviews (3)
Write a reviewHigh solubility. -
Good for intracellular staining. -
Effective in apoptosis assays. -
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