Active Recombinant Mouse Il1rn Protein (Arg27-Gln178), C-His tagged, Animal-free, Carrier-free

Cat.No. : Il1rn-56M
Product Overview : Recombinant Mouse Il1rn Protein (Arg27-Gln178) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.
  • Specification
  • Gene Information
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Description : Interleukin 1 receptor antagonist (IL-1RA) predicts a molecular mass of 19.8 kDa, is a member of the IL-1 family that binds to IL-1 receptors but does not induce any intracellular response. Two structural variants of IL-1RA have previously been described: a 17 kDa form that is secreted from monocytes, macrophages, neutrophils, and other cells (sIL-1RA) and an 18 kDa form that remains in the cytoplasm of keratinocytes and other epithelial cells, monocytes, and fibroblasts (icIL-1RA).
Source : E. coli
Species : Mouse
Tag : C-His
Form : Lyophilized
Bio-activity : Measure by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 cells. The ED50 for this effect is < 50 ng/mL.
Molecular Mass : The protein has a calculated MW of 18.27 kDa.
The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
Protein Length : Arg27-Gln178
Endotoxin : < 0.1 EU/μg of the protein by the LAL method.
Purity : >98% as determined by SDS-PAGE analysis.
Storage : This product is stable after storage at:
-20 centigrade for 12 months in lyophilized state from date of receipt.
-20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
Avoid repeated freeze/thaw cycles.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
Reconstitution : It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
Shipping : The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.
Gene Name : Il1rn interleukin 1 receptor antagonist [ Mus musculus (house mouse) ]
Official Symbol : Il1rn
Synonyms : IL1RN; interleukin 1 receptor antagonist; interleukin-1 receptor antagonist protein; IRAP; IL-1RN; IL1 inhibitor; IL-1ra; F630041P17Rik;
Gene ID : 16181
mRNA Refseq : NM_001039701
Protein Refseq : NP_001034790
UniProt ID : P25085

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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