Active Recombinant Mouse Il1rn Protein (Arg27-Gln178), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il1rn-56M
• Product Overview: Recombinant Mouse Il1rn Protein (Arg27-Gln178) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin 1 receptor antagonist (IL-1RA) predicts a molecular mass of 19.8 kDa, is a member of the IL-1 family that binds to IL-1 receptors but does not induce any intracellular response. Two structural variants of IL-1RA have previously been described: a 17 kDa form that is secreted from monocytes, macrophages, neutrophils, and other cells (sIL-1RA) and an 18 kDa form that remains in the cytoplasm of keratinocytes and other epithelial cells, monocytes, and fibroblasts (icIL-1RA).
• Source: E. coli
• Species: Mouse
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measure by its ability to inhibit IL-1 alpha -dependent proliferation in D10.G4.1 cells. The ED50 for this effect is < 50 ng/mL.
• Molecular Mass: The protein has a calculated MW of 18.27 kDa.
  The protein migrates as 17 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Arg27-Gln178
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il1rn interleukin 1 receptor antagonist [ Mus musculus (house mouse) ]
• Official Symbol: Il1rn
• Synonyms: IL1RN; interleukin 1 receptor antagonist; interleukin-1 receptor antagonist protein; IRAP; IL-1RN; IL1 inhibitor; IL-1ra; F630041P17Rik
• Gene ID: 16181
• mRNA Refseq: NM_001039701
• Protein Refseq: NP_001034790
• UniProt ID: P25085