Active Recombinant Mouse Il25 Protein (Val17-Ala169), C-His tagged, Animal-free, Carrier-free

• Cat.No.: Il25-23M
• Product Overview: Recombinant Mouse Il25 Protein (Val17-Ala169) with C-His tag was expressed in E. coli and Animal-free as well as Carrier-free.

Specification

• Description: Interleukin 25 (IL-25) is a cytokine with proinflammatory and anti-inflammatory effects, it predicts a molecular mass of 16.7 kDa. It can induce NF-κB activation, and stimulate the production of IL-8, which is the major chemotactic substance of neutrophils.
• Source: E. coli
• Species: Mouse
• Tag: C-His
• Form: Lyophilized
• Bio-activity: Measured by its ability to induce CXCL1 secretion in HT‑29 cells. The ED50 for this effect is < 1 ng/mL
• Molecular Mass: The protein has a calculated MW of 18.41kDa.
  The protein migrates about 17 kDa under reducing condition (SDS-PAGE analysis).
• Protein Length: Val17-Ala169
• Endotoxin: < 0.1 EU/μg of the protein by the LAL method.
• Purity: >98% as determined by SDS-PAGE analysis.
• Storage: This product is stable after storage at:
  -20 centigrade for 12 months in lyophilized state from date of receipt.
  -20 or -80 centigrade for 1 month under sterile conditions after reconstitution.
  Avoid repeated freeze/thaw cycles.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution containing 20 mM sodium citrate and 0.2 M NaCl, pH 4.5.
• Reconstitution: It is recommended to reconstitute the lyophilized protein in sterile H2O to a concentration not less than 200 μg/mL and incubate the stock solution for at least 20 min to ensure sufficient re-dissolved.
• Shipping: The product is shipped with polar packs. Upon receipt, store it immediately at -20 centigrade or lower for long term storage.

Gene Information

• Gene Name: Il25 interleukin 25 [ Mus musculus (house mouse) ]
• Official Symbol: Il25
• Synonyms: IL25; interleukin 25; interleukin-25; interleukin 17E; Il17e; IL-17e
• Gene ID: 140806
• mRNA Refseq: NM_080729
• Protein Refseq: NP_542767
• UniProt ID: Q8VHH8