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Active Recombinant Mouse Il27 protein, His-tagged

Cat.No. : Il27-1309M
Product Overview : Recombinant Mouse Il27(His193~Val296) fused with His tag at C-terminal was expressed in HEK293.
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Description : Recombinant Mouse Il27 complex composed of EBI3 (O35228) (Met1-Pro228) and IL27A (Q8K3I6) (Phe29-Ser234) linked by an polypeptide linker was fused with the polyhistidine tag at the C-terminus.
Source : HEK293
Species : Mouse
Tag : His
Predicted N Terminal : Tyr 19
Form : Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Bio-activity : 1. Measured by its binding ability in a functional ELISA. Immobilized mouse IL27-His at 10 μg/ml (100 μl/well) can bind human IL27RA-Fc, The EC50 of human IL27RA-Fc is 0.26-0.62 μg/ml.
2. Measured in antiviral assay using HepG2 cells infected with vesicular stomatitisvirus (VSV).
The ED50 for this effect is typically 0.07-0.42 μg/mL.
Molecular Mass : The recombinant mouse IL27 comprises 442 amino acids and has a predicted molecular mass of 49.5 kDa. The apparent molecular mass of the protein is approximately 60 kDa in SDS-PAGE under reducing conditions due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method
Purity : > 85 % as determined by SDS-PAGE
Stability : Samples are stable for up to twelve months from date of receipt at -70 centigrade.
Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Shipping : In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.
Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.
Gene Name : Il27 interleukin 27 [ Mus musculus ]
Official Symbol : Il27
Synonyms : IL27; interleukin 27; interleukin-27 subunit alpha; IL27-A; IL-27-A; interleukin 30; IL-27 p28 subunit; IL-27 subunit alpha; p28; Il30; IL-27; IL-27p28;
Gene ID : 246779
mRNA Refseq : NM_145636
Protein Refseq : NP_663611
UniProt ID : Q8K3I6
Chromosome Location : 7; 7 F3
Function : cytokine activity; interleukin-27 receptor binding; protein binding; receptor binding;

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (6)

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Are there have current difficulties in the study of Il27? 12/23/2022

The current challenges in the study of Il27 include potential side effects, preparation purity and other challenges.

Which scientific research fields are deeply studying Il27? 02/21/2022

The potential application of Il27 in tumor immunotherapy and autoimmune diseases is being deeply studied in the scientific research field.

Is the production of Il27 related to genetic factors? 09/19/2021

The production of Il27 is related to genetic factors, and the expression level and polymorphism of genes may affect its production and function.

Which role of Il27 in inflammatory diseases? 05/17/2021

Studies have shown that Il27 may have an anti-inflammatory effect in inflammatory diseases, regulating the degree of inflammatory response.

What the potential applications of recombinant Il27 in cancer therapy? 01/30/2021

Current studies have shown that Il27 has potential application value in enhancing anti-tumor immunity and alleviating tumor invasion and metastasis.

What is the effect of Il27 on immune response? 09/27/2020

Il27 can enhance the activation and proliferation of specific immune cells and regulate the intensity and direction of immune response.

Customer Reviews (3)

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Reviews
03/30/2022

    The purity is very high, no impurities.

    08/05/2020

      Biological activity is very strong and can be used in various biological experiments.

      05/30/2019

        Stability is very good, and it will not denaturate at high temperatures.

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