Active Recombinant Mouse Il4, Fc-tagged

• Cat.No.: Il4-316M
• Product Overview: The extracellular domain of mouse IL-4 (aa 21-140) is fused to the N-terminus of the Fc region of a mutant mouse IgG2a.

Specification

• Species: Mouse
• Source: CHO
• Tag: Fc
• Protein Length: 21-140 a.a.
• Description: Interleukin-4 (IL-4) is a cytokine produced by type 2 helper T cells, the Th2 cells. These cells tends to make a specific set of lymphokines including IL-4, IL-5, IL-6, IL-10, IL-13, IL-3 and GM-CSF and fail to produce IL-2, IFN-γ, and lymphotoxin (TNF-beta). In addition, mast cells can produce IL-4. IL-4 exerts numerous effects on various hematopoietic cell types. On B cells, IL-4 promotes immunoglobulin class switching to IgE and IgG1 isotypes and upregulates MHC class II and CD23 expression. IL-4 promotes survival, growth, and differentiation of both T and B lymphocytes, mast cells and endothelial cells. In addition, IL-4 inhibits the production of TNF, IL-1, and IL-6 by macrophages.
• Form: Lyophilized from 0.2μm-filtered solution in PBS.
• Bio-activity: Shows the biological function of the IL-4 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain.
• Purity: ≥98% (SDS-PAGE)
• Stability: Stable for at least 1 year after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.
• Storage: Short Term Storage: +4°C; Long Term Storage: -20°C. Avoid freeze/thaw cycles.

Gene Information

• Gene Name: Il4 interleukin 4 [ Mus musculus ]
• Official Symbol: Il4
• Synonyms: IL4; interleukin 4; interleukin-4; IGG1 induction factor; B-cell growth factor 1; B-cell stimulatory factor 1; lymphocyte stimulatory factor 1; B-cell IgG differentiation factor; Il-4; BSF-1
• Gene ID: 16189
• mRNA Refseq: NM_021283
• Protein Refseq: NP_067258
• Pathway: Allograft rejection, organism-specific biosystem; Allograft rejection, conserved biosystem; Asthma, organism-specific biosystem; Asthma, conserved biosystem; Autoimmune thyroid disease, organism-specific biosystem; Autoimmune thyroid disease, conserved biosystem; Cytokine-cytokine receptor interaction, organism-specific biosystem
• Function: cytokine activity; cytokine receptor binding; growth factor activity; interleukin-4 receptor binding

Citation

Altered expression of the immunoregulatory ligand-receptor pair CD200-CD200R1 in the brain of Parkinson’s disease patients

Journal: NPJ Parkinson's Disease    PubMed ID: 35296683    Data: 2022/3/16

Authors: Neus Rabaneda-Lombarte, José Manuel Vidal-Taboada, Carme Solà

Article Snippet:Apart from expressing classical myeloid markers (PU.1 and Iba1) and microglial specific markers (TMEM119 and P2YR12), they show reduced expression of CD45, CD14, and CD200R when compared to human monocyte-derived macrophages and decreased CCR2 when compared to human monocytes and similar responses to IL-4 and dexamethasone than authentic microglia , .myeloid markers (PU.1 and Iba1) and microglial specific markers (TMEM119 and P2YR12), they show reduced expression of CD45, CD14, and CD200R when compared to human monocyte-derived macrophages and decreased CCR2 when compared to human monocytes and similar responses to IL-4 and dexamethasone than authentic microglia , . ... Cells were treated with LPS from Escherichia coli (100 ng/mL; 026:B6; Sigma-Aldrich) or IL4 (50 ng/mL; recombinant mouse IL4 expressed in CHO cells, Creative BioMart, Shirley, NY, USA) and processed after 24 h. Control and treated cells were derived from the same donor.. A stock solution of 1 mg/mL LPS in a serum-free culture medium was prepared and stored at ?20 °C.A stock solution of 1 mg/mL LPS in a serum-free culture medium was prepared and stored at ?20 °C.

mRNA expression of the four CD200R1 mRNA variants in microglia-like cells treated for 24 h with LPS (100 ng/mL) ( a ) or IL4 (50 ng/mL) ( b ) by qRT-PCR after gene-specific retrotranscription. GAPDH and RPS18 were used as reference genes. Individual points are represented and bars indicate the positions of the mean ± SEM of 3–4 independent experiments. * p < 0.05 and ** p < 0.01; two-tailed paired Student’s t -test.