Active Recombinant Mouse Interleukin 1 Receptor, Type I, Fc-tagged

• Cat.No.: Il1r1-5653M
• Product Overview: RecombinantMouse Il1r1 protein was expressed in Murine myeloma celllinewith the Fc part of Human IgG1 Pro100-Lys330 (C-terminus) and MouseIl1r1 Leu20-Lys338 (N-terminus).

Specification

• Species: Mouse
• Source: Mammalian Cells
• Tag: Fc
• Protein Length: 20-338 a.a.
• Description: Il1r1 isan 80 kDa transmembrane protein that is expressed predominantly by T cells,fibroblasts, and endothelialcells. IL-1 receptor Type II is a 68 kDatransmembrane protein found on B lymphocytes, neutrophils, monocytes, largegranular leukocytes and endothelial cells. Both receptors are members of theimmunoglobulin superfamily and show approximately 28% sequence identity intheir extracellular domains. The two receptor types do not heterodimerize intoa receptor complex. Mouse Il1r1 shares 63% amino acid sequence homology withhuman Il1r1 in their extracellular domains.
• Form: Lyophilizedfrom a 0.2 um filtered solution in PBS.
• N-terminalSequence: Leu20
• MolecularWeight: 63.8 kDa (monomer)
• Activity: Measuredby its ability to inhibit IL-1α-dependent proliferation in D10.G4.1 mouse helperT cells. Approximately 0.005-0.015 μg/mL of IL-1 sRI/Fc will inhibit 50% ofthe biological response due to 50 pg/mL of rmIL-1a.
• Endotoxin Level: <1.0EU per 1μg of the protein by the LAL method.
• SDS-PAGE: 95-110kDa, reducing conditions.
• Purity: >90%,by SDS-PAGE under reducing conditions and visualized by silver stain.
• Reconstitution: 100 μg/mLin sterile PBS
• Storage: Use amanual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70°C as supplied.1 month, 2 to 8°C under sterile conditions after reconstitution. 3months, -20 to -70°C under sterile conditions after reconstitution.

Gene Information

• Gene Name: Il1r1 interleukin 1 receptor,type I [ Mus musculus ]
• Official Symbol: Il1r1
• Synonyms: Il1r1; interleukin 1receptor, type I; IL-iR; CD121a;CD121b; Il1r-1; interleukin-1 receptor type 1; p80; IL-1R-1; IL-1RT1;IL-1RT-1; IL-1R-alpha; IL-1 receptor alpha chain; interleukin-1 receptoralpha; interleukin-1 receptor type I; CD121 antigen-like family member A
• Gene ID: 16177
• mRNA Refseq: NM_001123382
• Protein Refseq: NP_001116854
• UniProt ID: Q4FK69
• Chromosome Location: 1
• Pathway: Amoebiasis;Apoptosis; Cytokine-cytokine receptor interaction; HTLV-I infection;Hematopoietic cell lineage; Hypertrophy Model; IL-1 Signaling Pathway; MAPKsignaling pathway; Osteoclast differentiation
• Function: interleukin-1receptor activity; interleukin-1, Type I, activating receptor activity;ionotropic glutamate receptor binding; platelet-derived growth factorreceptor binding; protease binding; protein binding; receptor activity;signal transducer activity; transmembrane signaling receptor activity