Active Recombinant Mouse Interleukin-2, His Tag

• Cat.No.: IL2-403M
• Product Overview: Interleukin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 170 amino acids (21-169 a.a) and having a molecular mass of 19.5kDa. The mouse IL-2 is fused to a 20 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

Specification

• Species: Mouse
• Source: Mouse
• Tag: His
• Protein Length: 21-169 a.a.
• Description: IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
• Form: The Mouse IL2 protein (0.5mg/ml) is supplied in Phosphate Buffered Saline (pH7.4) 0.1M NaCl, and 20% glycerol.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Bio-activity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 2.5 ng/ml, corresponding to a specific activity of less than 400,000units/mg.
• Physical Appearance: Sterile Filtered colorless clear solution.
• Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSVISTSPQ.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

Gene Information

• Gene Name: Il2 interleukin 2 [ Mus musculus ]
• Official Symbol: IL2
• Synonyms: IL-2; TCGF; lymphokine; T-cell growth factor; Aldesleukin, Interleukin-2; interleukin-2 precursor; involved in regulation of T-cell clonal expansion; IL2; interleukin 2
• Gene ID: 16183
• mRNA Refseq: NM_008366
• Protein Refseq: NP_032392
• UniProt ID: P04351
• Chromosome Location: 3 B-C; 3 19.2 cM
• Pathway: Allograft rejection; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Jak-STAT signaling pathway; T cell receptor signaling pathway; Type I diabetes mellitus
• Function: cytokine activity; growth factor activity; interleukin-2 receptor binding; protein binding