Active Recombinant Mouse Interleukin-2, His Tag

• Cat.No.: IL2-403M
• Product Overview: Interleukin-2 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 170 amino acids (21-169 a.a) and having a molecular mass of 19.5kDa. The mouse IL-2 is fused to a 20 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.

Specification

• Species: Mouse
• Source: Mouse
• Tag: His
• Protein Length: 21-169 a.a.
• Description: IL2 is a secreted cytokine that is important for the proliferation of T and B lymphocytes. The receptor of this cytokine is a heterotrimeric protein complex whose gamma chain is also shared by interleukin 4 (IL4) and interleukin 7 (IL7). The expression of this gene in mature thymocytes is monoallelic, which represents an unusual regulatory mode for controlling the precise expression of a single gene. The targeted disruption of a similar gene in mice leads to ulcerative colitis-like disease, which suggests an essential role of this gene in the immune response to antigenic stimuli.
• Form: The Mouse IL2 protein (0.5mg/ml) is supplied in Phosphate Buffered Saline (pH7.4) 0.1M NaCl, and 20% glycerol.
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Bio-activity: Measured in a cell proliferation assay using CTLL2 mouse cytotoxic T cells. The ED50 for this effect is < 2.5 ng/ml, corresponding to a specific activity of less than 400,000units/mg.
• Physical Appearance: Sterile Filtered colorless clear solution.
• Amino acid sequence: MGSSHHHHHH SSGLVPRGSH MAPTSSSTSS STAEAQQQQQ QQQQQQQHLE QLLMDLQELL SRMENYRNLK LPRMLTFKFY LPKQATELKD LQCLEDELGP LRHVLDLTQS KSFQLEDAEN FISNIRVTVV KLKGSDNTFE CQFDDESATV VDFLRRWIAF CQSVISTSPQ.
• Storage: Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Please avoid freeze thaw cycles.

Gene Information

• Gene Name: Il2 interleukin 2 [ Mus musculus ]
• Official Symbol: IL2
• Synonyms: IL-2; TCGF; lymphokine; T-cell growth factor; Aldesleukin, Interleukin-2; interleukin-2 precursor; involved in regulation of T-cell clonal expansion; IL2; interleukin 2
• Gene ID: 16183
• mRNA Refseq: NM_008366
• Protein Refseq: NP_032392
• UniProt ID: P04351
• Chromosome Location: 3 B-C; 3 19.2 cM
• Pathway: Allograft rejection; Autoimmune thyroid disease; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Jak-STAT signaling pathway; T cell receptor signaling pathway; Type I diabetes mellitus
• Function: cytokine activity; growth factor activity; interleukin-2 receptor binding; protein binding

Citation

Interaction of matrix metalloproteinase-9 and Zpx in Cronobacter turicensis LMG 23827 T mediated infections in the zebrafish model.

Journal: Cellular microbiology    PubMed ID: 29972620    Data: 2019/9/23

Authors: Athmanya Konegadde Eshwar, Nina Wolfrum, Angelika Lehner

Article Snippet:After a second centrifugation step, the cells were re-suspended in DPBS, and appropriate dilutions were prepared in DPBS.After a second centrifugation step, the cells were re-suspended in DPBS, and appropriate dilutions were prepared in DPBS.. In vitro Western blot analysis Forty ng of recombinant human MMP-9 with a C-terminal His-tag (Creative BioMart) was incubated for 2.5 h at 37 °C with 104 CFU of the respective bacteria in PBS (20 μl total volume).. The bacteria-containing samples were centrifuged for 10 min at 3000 x g and the supernatants were mixed with 5 μl 5x non-reducing sample buffer (8 % SDS, 40 % glycerol, 0.02 % bromophenol blue, 250 mM TrisHCl, pH 6.8), separated on a 7.5 % SDS-PAGE and transfer to a 0.45 μm nitrocellulose membrane (Amersham, GE Healthcare, NJ, USA) by semi-dry blotting.The bacteria-containing samples were centrifuged for 10 min at 3000 x g and the supernatants were mixed with 5 μl 5x non-reducing sample buffer (8 % SDS, 40 % glycerol, 0.02 % bromophenol blue, 250 mM TrisHCl, pH 6.8), separated on a 7.5 % SDS-PAGE and transfer to a 0.45 μm..

An alternative view of the proposed alternative activities of hemopexin

Journal: Protein Science : A Publication of the Protein Society    PubMed ID: 21404362    Data: 2011/5/1

Authors: Marcia R Mauk, Ann Smith, A Grant Mauk

Article Snippet:Although recombinant human hemopexin expressed in P. pastoris has been used in at least one report, 23 no evidence has been provided to establish that this protein is properly folded.Although recombinant human hemopexin expressed in P. pastoris has been used in at least one report, 23 no evidence has been provided to establish that this protein is properly folded.. Commercially available recombinant human hemopexin (e.g., R&D Systems, Minneapolis, MN; Creative BioMart, Shirley, NY; Sino Biological, Beijing, China) has the potential complication of a His-tag, which in the absence of information to the contrary may bind heme or may promote oligomerization of the protein.. 38 , 39 Little or no functional evidence is provided by the vendors to validate the structural authenticity of their recombinant protein.38 , 39 Little or no functional evidence is provided by the vendors to validate the structural authenticity of their recombinant protein.