Active Recombinant Mouse Interleukin 22, PEG-tagged
Cat.No. : | Il22-439M |
Product Overview : | Pegylated Interleukin-22 Mouse Recombinant produced in E.Coli is a single, non-glycosylated homodimeric polypeptide chain containing 147 amino acids and an aditionalAla amino acid at N-terminus having a molecular mass of 36 kDa as determioned by mass spectometry. However due to enlargedhydrodymanic volume it runs on the SDS-PAGE as a 50 kDa protein and in gel-filtration on Superdex 200 as over 200 kDa protein. The Murine IL-22 is Mono-pegylated (with 20 kDa PEG) purified by proprietary chromatographic techniques. |
- Specification
- Gene Information
- Related Products
Cat. No. : | Il22-439M |
Description : | Interleukin-22 (IL-22), also known as IL-10-related T cell-derived inducible factor (IL-TIF) was initially identified as a gene induced by IL-9 in mouse T cells and mast cells. Mouse IL-22 cDNA encodes a 179 amino acid residue protein with a putative 33 amino acids signal peptide that is cleaved to generate a 147 aa mature protein that shares approximately 79% and 22% aa sequence identity with human IL-22 and IL-10, respectively. IL-22 has been shown to activate STAT-1 and STAT-3 in several hepatoma cell lines and upregulate the production of acute phase proteins. IL-22 is produced by normal mouse T cells upon Con A activation. |
Source : | Mouse |
Host : | Escherichia Coli. |
Form : | Lyophilized from a concentrated solution at 0.65mg/ml containing 0.003mM NaHCO3. |
Purity : | Greater than 98.0% as determined by: (a) Analysis by Gel-Filtration. (b) Analysis by SDS-PAGE. |
Bio-activity : | The ED50 as determined by STAT3 phosphorylation assay in HepG cells. The activity in vitro was found to be ~ 10% compared to the non-pegylated mouse IL22. |
Physical Appearance : | Sterile Filtered White lyophilized (freeze-dried) powder. |
Solubility : | It is recommended to reconstitute the lyophilized pegylated mouse Interleukin -22 in sterile 0.4% NaHCO3 adjusted to pH 8-9 not less than 100µg/ml, which can then be further diluted to other aqueous solutions. |
Storage : | Lyophilized pegylated murine IL22 although stable at room temperature for several days, should be stored desiccated below -20°C. Upon reconstitution at 0.1mg/ml pegylated mouse IL22 and up to 2mg/ml, filter and sterilized, the protein can be stored at 4 degrees Celsius for several weeks making it suitable for long term infusion studies using osmotic pumps. At lower concentration addition of a carrier protein (0.1% HSA or BSA) is suggested. Please prevent freeze-thaw cycles. |
Pathways : | Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway |
Gene Name : | Il22 interleukin 22 [ Mus musculus ] |
Official Symbol : | Il22 |
Synonyms : | IL-22, IL-TIF, ILTIF, TIFa, IL-10-related T-cell-derived-inducible factor, ILTIFA, IL-D110, zcyto18, MGC79382, MGC79384, TIFIL-23, Interleukin-22, IL-TIF alpha, Interleukin-22a, IL-22a, Interleukin-22 Precursor |
Gene ID : | 50929 |
mRNA Refseq : | NM_016971 |
Protein Refseq : | NP_058667 |
UniProt ID : | Q9JJY9 |
Chromosome Location : | 10 D2; 10 67.0 cM |
Function : | cytokine activity; cytokine receptor binding |
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◆ Lysates | ||
IL22-5232HCL | Recombinant Human IL22 293 Cell Lysate | +Inquiry |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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