Active Recombinant Mouse Itgam&Itgb2

• Cat.No.: Itgam&Itgb2-605M
• Product Overview: Recombinant Mouse Itgam&Itgb2 (Accession # NP_032427 (Integrin alpha M) & P11835 (Integrin beta 2)), fused with , was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO Cells
• Species: Mouse
• Predicted N Terminal: Phe17 (Integrin alpha M) & Gln24 predicted, No results obtained: sequencing might be blocked (Integrin beta 2)
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of CHO Chinese hamster ovary cells transfected with ICAM-1.The ED50 for this effect is typically 0.4-1.6 µg/mL.
• Molecular Mass: Recombinant Mouse Itgam&Itgb2 has a calculated MW of 130 kDa (Integrin alpha M) & 83 kDa (Integrin beta 2). In SDS-PAGE migrates as 155-180 kDa & 95-105 kDa, reducing conditions.
• Purity: >95%, by SDS-PAGE with silver staining.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Itgam integrin alpha M [ Mus musculus ]
• Official Symbol: Itgam
• Synonyms: ITGAM; integrin alpha M; integrin alpha-M; Mac-1 alpha; CD11B (p170); CR-3 alpha chain; macrophage antigen alpha; complement receptor type 3; leukocyte adhesion receptor MO1; CD11 antigen-like family member B; complement component receptor 3 alpha-a; cell surface glycoprotein MAC-1 alpha subunit; cell surface glycoprotein MAC-1 subunit alpha; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik
• Gene ID: 16409
• mRNA Refseq: NM_001082960
• Protein Refseq: NP_001076429