Active Recombinant Mouse Jam2 protein, hFc-tagged

• Cat.No.: Jam2-909M
• Product Overview: Recombinant Mouse Jam2 protein(NP_076333.3)(Met1-Asn236), fused with hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Asn236
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human leukemic T cells. When 8 x 10E4 cells/well are added to JAM2-coated plates (0.2 μg/ml and 100 μl/well), approximately 40-55% will adhere specifically after 60 minutes at 37℃.
• Molecular Mass: The secreted recombinant mouse JAM-2/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 449 amino acids and has a predicted molecular mass of 50.3 kDa. rm JAM-2/Fc monomer migrates as an approximately 65 kDa band in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 70 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Jam2 junction adhesion molecule 2 [ Mus musculus ]
• Official Symbol: Jam2
• Synonyms: JAM2; junction adhesion molecule 2; junctional adhesion molecule B; junction adhesion molecule B; junctional adhesion molecule 2; junction cell adhesion molecule 2; vascular endothelial junction-associated molecule; JAM-2; JAM-B; Jcam2; VE-JAM; 1110002N23Rik; 2410030G21Rik; 2410167M24Rik
• Gene ID: 67374
• mRNA Refseq: NM_023844
• Protein Refseq: NP_076333