Active Recombinant Mouse KLB protein, His-tagged

• Cat.No.: KLB-0319M
• Product Overview: Recombinant Mouse KLB protein(Phe53-Leu995), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Human FGF19, Fc Tag on Protein A Biosensor, can bind Mouse Klotho beta, His Tag with an affinity constant of 78.6 nM as determined in BLI assay (ForteBio Octet Red96e).Loaded Human FGF R4, Fc Tag on Protein A Biosensor, can bind Mouse Klotho beta, His Tag with an affinity constant of 54.0 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 110.8 kDa. The protein migrates as 125-145 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe53-Leu995
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >90% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Klb klotho beta [ Mus musculus ]
• Official Symbol: KLB
• Synonyms: KLB; klotho beta; beta-klotho; BKL; klotho beta-like protein; AV071179; betaKlotho
• Gene ID: 83379
• mRNA Refseq: NM_031180
• Protein Refseq: NP_112457