Active Recombinant Rhesus macaque KLB protein, His-tagged

• Cat.No.: KLB-1291R
• Product Overview: Recombinant Rhesus macaque KLB protein(Phe 53 - Leu 997), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rhesus macaque
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Human FGF R4, Fc Tag on Protein A Biosensor, can bind Rhesus macaque Klotho beta, His Tag with an affinity constant of 0.9 μM as determined in BLI assay (ForteBio Octet Red96e).Loaded Human FGF19, Fc Tag on Protein A Biosensor, can bind Rhesus macaque Klotho beta, His Tag with an affinity constant of 78.5 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 110.3 kDa. The protein migrates as 115-130 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Phe53-Leu997
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.