Active Recombinant Mouse lgfbp4 protein, His-tagged
Cat.No. : | lgfbp4-760M |
Product Overview : | Recombinant Mouse lgfbp4 protein(NP_034647.1)(Met1-Glu254), fused with His tag, was expressed in HEK293. |
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Source : | HEK293 |
Species : | Mouse |
Tag : | His |
Form : | Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. |
Bio-activity : | Measured in a serum free cell proliferation assay using MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. 1988, Cancer Research 48: 4083.). The ED50 for this effect is typically 0.1-0.6 μg/ml. |
Molecular Mass : | The secreted recombinant mouse IGFBP4 consists of 244 amino acids and has a calculated molecular mass of 27.2 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 38 kDa protein in SDS-PAGE under reducing conditions. |
Protein Length : | Met1-Glu254 |
Endotoxin : | < 1.0 EU per μg of the protein as determined by the LAL method |
Purity : | > 95 % as determined by SDS-PAGE |
Storage : | Samples are stable for up to twelve months from date of receipt at -20°C to -80°C Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. |
Reconstitution : | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. |
Official Symbol : | lgfbp4 |
Official Symbol : | lgfbp4 |
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For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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