Active Recombinant Mouse Lxn protein, His-tagged

• Cat.No.: Lxn-3998M
• Product Overview: Recombinant Mouse Lxn protein(NP_058033.2)(Glu2-Glu222), fused with His tag, was expressed in E. coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: Glu2-Glu222
• Form: Lyophilized from sterile PBS, pH 8.0, 10% glycerolPlease contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to inhibit carboxypeptidase-A1 cleavage of the colorimetric peptide substrate Ac-Phe-Thiaphe-OH in the presence of 5,5'Dithiobis(2-nitrobenzoic acid) (DTNB) (Edwards, K.M. et al, 1999, J. Biol. Chem. 274:30468). The IC50 value is <2.0 nM .
• Molecular Mass: The recombinant mouse LXN consisting of 228 amino acids and has a calculated molecular mass of 26.3 kDa. The apparent molecular mass of the rmLXN is approximately 32 kDa in SDS-PAGE under reducing conditions.
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Lxn latexin [ Mus musculus ]
• Official Symbol: Lxn
• Synonyms: LXN; latexin; ECI; TCI; tissue carboxypeptidase inhibitor; endogenous carboxypeptidase inhibitor; MGC144352; MGC144353
• Gene ID: 17035
• mRNA Refseq: NM_016753
• Protein Refseq: NP_058033