Active Recombinant Mouse NEC protein, His-tagged, low endotoxin

• Cat.No.: NEC-0012M
• Product Overview: Recombinant Mouse NEC protein(Gly 31 - Ser 347), fused with His tag at the C-terminus, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Gly 31 - Ser 347
• Form: Lyophilized from 0.22 μm filtered solution in PBS, Arginine, pH7.4. Normally trehalose is added as protectant before lyophilization.
• Bio-activity: Loaded Mouse Nectin-4, His Tag on NTA Biosensor, can bind Human Nectin-1, Fc Tag with an affinity constant of 48.8 nM as determined in BLI assay (ForteBio Octet Red96e).
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminus.The protein has a calculated MW of 36.2 kDa. The protein migrates as 38-43 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.