Active Recombinant Mouse Ngf Protein, Tag Free

• Cat.No.: Ngf-11198M
• Product Overview: Mouse Beta-NGF Protein, Tag Free is expressed from human 293 cells (HEK293). It contains AA Ser 122-Gly 241 (Accession # P01139). This protein carries no "tag". Predicted N-terminus: Ser 122

Specification

• Species: Mouse
• Source: HEK293
• Tag: Non
• Protein Length: 122-241 aa
• Description: Nerve growth factor beta (beta-NGF) is a neurotrophic factor that plays critical role in the development and maintenance of sensory and sympathetic neurons. The active form of human beta-NGF is is a noncovalently disulfide-linked homodimer, which is secreted and signals through the low affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase A (TrkA) to activate PI3K, Ras, and PLC signaling pathways.
• Form: Lyophilized from 0.22 μm filtered solution in 20 mM NaAC, 150 mM NaCl, pH5.5 with trehalose as protectant.
• Specificity: Sterility: Negative Mycoplasma: Negative
• Bio-activity: Immobilized Mouse Beta-NGF Protein, Tag Free at 1 μg/mL (100 μL/well) can bind Human TrkA (33-417), Fc Tag with a linear range of 0.2-16 ng/mL.
• Molecular Mass: The protein has a calculated MW of 13.5 kDa. The protein migrates as <14 kDa under reducing (R) condition (SDS-PAGE).
• Endotoxin: Less than 0.1 EU/μg by the LAL method/rFC method.
• Purity: >95% as determined by SDS-PAGE. >90% as determined by SEC-MALS.
• Storage: This product is stable after storage at: -20 to -70 centigrade for 12 months in lyophilized state; -70 centigrade for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20 centigrade or lower. Please avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 μg/μL. Centrifuge the vial at 4 centigrade before opening to recover the entire contents.

Gene Information

• Gene Name: Ngf nerve growth factor [ Mus musculus (house mouse) ]
• Official Symbol: Ngf
• Synonyms: NGF; nerve growth factor; beta-nerve growth factor; beta-NGF; nerve growth factor, beta; Ngfb
• Gene ID: 18049
• mRNA Refseq: NM_001112698
• Protein Refseq: NP_001106168
• UniProt ID: P01139