Active Recombinant Mouse PDCD1 protein, mFc-tagged

• Cat.No.: PDCD1-369M
• Product Overview: Recombinant Mouse PDCD1 protein(Ser 21 - Gln 167), fused with mFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: mFc
• Protein Length: Ser 21 - Gln 167
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Mouse PD-L1, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse PD-1, Mouse IgG2a Fc Tag with a linear range of 0.078-0.313 μg/mL. Immobilized Mouse PD-L2, Fc Tag at 5 μg/mL (100 μL/well) can bind Mouse PD-1, Mouse IgG2a Fc Tag with a linear range of 0.078-0.625 μg/mL.
• Molecular Mass: This protein carries a mouse IgG2a Fc tag at the C-terminusThe protein has a calculated MW of 43.7 kDa. The protein migrates as 55-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Endotoxin: Less than 0.1 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: For long term, the product should be stored at lyophilized state at -20°C or lower.Please avoid repeated freeze-thaw cycles. This product is stable after at:
  -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Pdcd1 programmed cell death 1 [ Mus musculus ]
• Official Symbol: PDCD1
• Synonyms: PDCD1; programmed cell death 1; programmed cell death protein 1; mPD-1; protein PD-1; PD-1; Pdc1; Ly101
• Gene ID: 18566
• mRNA Refseq: NM_008798
• Protein Refseq: NP_032824