Active Recombinant Mouse Pdgfa

• Cat.No.: Pdgfa-304M
• Product Overview: PDGF-AA Mouse Recombinant is a disulfide linked homodimeric, non-glycosylated, polypeptide chain containing 2 x 126 amino acids and having a total molecular mass of 28.9 kDa. PDGF-AA is purified by proprietary chromatographic techniques.

Specification

• Species: Mouse
• Source: E.coli
• Tag: Non
• Description: PDGF-AA, PDGF-BB and PDGF-AB, are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGF is stored in platelet alpha-granules and released upon platelet activation. The PDGF is involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGF have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB.
• Form: Sterile Filtered White lyophilized (freeze-dried) powder. The protein was lyophilized with no additives.
• Bio-activity: Established by the dose-dependent stimulation of Balb/c 3T3 cells proliferation. The expected ED50 for this effect is 8-10 ng/ml corresponding to a specific activity of 100,000-125,000IU/mg.
• AA Sequence: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATSNLN PDHREEETGR RRESGKNRKR KRLKPT.
• Purity: Greater than 97.0% as determined by RP-HPLC and SDS-PAGE.
• Storage: Lyophilized PDGF-AA although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution PDGF-AA should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
• Publications:
  MiR-34a regulates Migration of Mouse Lung Fibroblasts by modulating PDGFR signalling (2019)

Gene Information

• Gene Name: Pdgfa platelet derived growth factor, alpha [ Mus musculus (house mouse) ]
• Official Symbol: Pdgfa
• Synonyms: Pdgfa; PDGF-1; platelet derived growth factor, alpha; platelet-derived growth factor subunit A; PDGF subunit A; platelet-derived growth factor A chain; platelet-derived growth factor alpha polypeptide
• Gene ID: 18590
• mRNA Refseq: NM_008808
• Protein Refseq: NP_032834
• UniProt ID: P20033
• Chromosome Location: 5 G2; 5 77.65 cM
• Pathway: Adaptive Immune System; Cytokine-cytokine receptor interaction; Cytokines and Inflammatory Response (BioCarta)
• Function: growth factor activity; platelet-derived growth factor binding; platelet-derived growth factor receptor binding