Active Recombinant Mouse Pdgfrb Protein, His-Tagged

• Cat.No.: Pdgfrb-01M
• Product Overview: Recombinant Mouse Pdgfrb Protein, His-Tagged was expressed in Mouse myeloma cell line cell

Specification

• Description: Enables platelet-derived growth factor-activated receptor activity and signaling receptor binding activity. Involved in several processes, including positive regulation of intracellular signal transduction; protein phosphorylation; and vasculature development. Acts upstream of or within several processes, including animal organ development; response to ceramide; and ruffle assembly. Located in cell surface; cytoplasm; and ruffle. Is expressed in several structures, including alimentary system; extraembryonic component; genitourinary system; nervous system; and sensory organ. Used to study basal ganglia calcification; infantile myofibromatosis; myeloproliferative neoplasm; and severe nonproliferative diabetic retinopathy. Human ortholog(s) of this gene implicated in basal ganglia calcification; glioblastoma; infantile myofibromatosis; myeloproliferative neoplasm (multiple); and renal cell carcinoma. Orthologous to human PDGFRB (platelet derived growth factor receptor beta).
• Source: Mouse myeloma cell line
• Species: Mouse
• Tag: His
• Form: Lyophilized
• Bio-activity: Measured by its binding ability in a functional ELISA. When Recombinant Rat PDFG-BB is immobilized at 2 µg/mL (100 µL/well), the concentration of Recombinant Mouse PDGF R beta that produces 50% of the optimal binding response is 0.25-1.5 μg/mL.
• Molecular Mass: 57 kDa
• N-terminal Sequence Analysis: Leu32
• Endotoxin: <0.10 EU per 1 μg of the protein by the LAL method.
• Purity: >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 °C as supplied.
  1 month, 2 to 8 °C under sterile conditions after reconstitution.
  3 months, ≤ -20 °C under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.
• Shipping: The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Gene Information

• Gene Name: Pdgfrb platelet derived growth factor receptor, beta polypeptide [ Mus musculus (house mouse) ]
• Official Symbol: Pdgfrb
• Synonyms: Pdgfr; CD140b; PDGFR-1
• Gene ID: 18596
• mRNA Refseq: NM_001146268.1
• Protein Refseq: NP_001139740.1
• UniProt ID: P05622