| Description : |
Enables mRNA binding activity and pyruvate kinase activity. Involved in positive regulation of cytoplasmic translation. Acts upstream of or within glycolytic process. Located in cilium; photoreceptor inner segment; and rough endoplasmic reticulum. Is expressed in several structures, including alimentary system; genitourinary system; nervous system; respiratory system; and sensory organ. Orthologous to human PKM (pyruvate kinase M1/2). |
| Source : |
E. coli |
| Species : |
Mouse |
| Tag : |
N-6×His |
| Form : |
Liquid |
| Bio-activity : |
Specific activity: > 50,000 pmol/min/μg. One unit will convert 1.0 pmole of phospho(enol)pyruvate to pyruvate per minute at pH 7.5 at 37 centigrade. |
| Molecular Mass : |
60.2 kDa (554aa) Confirmed by MALDI-TOF |
| Protein Length : |
1-531 |
| AA Sequence : |
MGSSHHHHHHSSGLVPRGSHMGSMP KPHSEAGTAFIQTQQLHAAMADTFL EHMCRLDIDSAPITARNTGIICTIG PASRSVEMLKEMIKSGMNVARLNFS HGTHEYHAETIKNVREATESFASDP ILYRPVAVALDTKGPEIRTGLIKGS GTAEVELKKGATLKITLDNAYMEKC DENILWLDYKNICKVVEVGSKIYVD DGLISLQVKEKGADFLVTEVENGGS LGSKKGVNLPGAAVDLPAVSEKDIQ DLKFGVEQDVDMVFASFIRKAADVH EVRKVLGEKGKNIKIISKIENHEGV RRFDEILEASDGIMVARGDLGIEIP AEKVFLAQKMMIGRCNRAGKPVICA TQMLESMIKKPRPTRAEGSDVANAV LDGADCIMLSGETAKGDYPLEAVRM QHLIAREAEAAIYHLQLFEELRRLA PITSDPTEAAAVGAVEASFKCCSGA IIVLTKSGRSAHQVARYRPRAPIIA VTRNPQTARQAHLYRGIFPVLCKDA VLNAWAEDVDLRVNLAMDVGKARGF FKKGDVVIVLTGWRPGSGFTNTMRV VPVP |
| Purity : |
>85% by SDS-PAGE |
| Applications : |
Enzyme Activity, SDS-PAGE |
| Storage : |
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
0.5 mg/mL (determined by absorbance at 280nm) |
| Storage Buffer : |
20mM Tris-HCl buffer (pH8.5) containing 0.2M NaCl, 1mM DTT, 30% glycerol |
