Active Recombinant Mouse Prss2 protein, His-tagged

• Cat.No.: Prss2-909M
• Product Overview: Recombinant Mouse Prss2 protein(NP_033456.1)(Met1-Asn246), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Asn246
• Form: Lyophilized from sterile PBS, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2. The specific activity is >1500 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)
• Molecular Mass: The recombinant pro form of mouse PRSS2 comprises 242 amino acids and has a predicted molecular mass of 26.2 kDa. The apparent molecular mass of rm PRSS2 is approximately 32 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 92 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Prss2 protease, serine, 2 [ Mus musculus ]
• Official Symbol: Prss2
• Synonyms: PRSS2; protease, serine, 2; anionic trypsin-2; trypsin 2; trypsinogen 20; pretrypsinogen II; serine protease 2; anionic trypsin II; Ta; TRY8; TRYP; Try2; Tesp4
• Gene ID: 22072
• mRNA Refseq: NM_009430
• Protein Refseq: NP_033456