Active Recombinant Mouse Sema3c Protein, His&Fc tagged

• Cat.No.: Sema3c-903M
• Product Overview: Recombinant mouse Semaphorin 3C (Gln24-Ser741, Arg548Ala, Arg552Ala) with Human IgG1 (Pro100-Lys330) Fc tag at C-terminus ant His tag at N-terminus was expressed in Spodoptera frugiperda, Sf 21 (baculovirus).

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: Fc&His
• Description: Enables semaphorin receptor binding activity. Involved in several processes, including cardiac endothelial to mesenchymal transition; chordate embryonic development; and circulatory system development. Acts upstream of or within several processes, including blood vessel remodeling; dichotomous subdivision of terminal units involved in salivary gland branching; and neural crest cell migration. Located in extracellular space. Is expressed in several structures, including alimentary system; central nervous system; genitourinary system; heart; and respiratory system. Orthologous to human SEMA3C (semaphorin 3C).
• Form: Disulfide-linked homodimer, Lyophilized from a 0.2 μm filtered solution in Tris, Citric Acid, NaCl and Tween 20.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of SVEC4-10 mouse vascular endothelial cells. The ED50 for this effect is 0.12-1.2 μg/mL.
• Molecular Mass: 108.9 kDa
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Shipping: The product is shipped at ambient temperature.
• Reconstitution: Reconstitute at 100 μg/mL in sterile PBS.
• N-terminal Sequence Analysis: Met

Gene Information

• Gene Name: Sema3c sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C [ Mus musculus (house mouse) ]
• Official Symbol: Sema3c
• Synonyms: Sema3c; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3C; SemE; Semae; 1110036B02Rik; semaphorin-3C; sema E; semaphorin E
• Gene ID: 20348
• mRNA Refseq: NM_013657
• Protein Refseq: NP_038685
• UniProt ID: Q62181