Active Recombinant Mouse Sema5b, Fc Chimera

• Cat.No.: Sema5b-337M
• Product Overview: Recombinant Mouse Sema5b (Accession # AAH52397) was produced in Chinese Hamster Ovary cell line, CHO-derived.

Specification

• Source: CHO cells
• Species: Mouse
• Tag: Fc Chimera
• Predicted N Terminal: No results obtained, sequencing might be blocked: Gln27 is predicted
• Form: Lyophilized from a 0.2 µm filtered solution in PBS.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of human pancreatic cancer cells.When 5 x 10^4 cells/well are added to Recombinant Mouse Semaphorin 5B Fc Chimera coated plates, cell adhesion is enhanced in a dose dependent manner after 1 hour at 37 °C. The ED50 for this effect is typically 1.0 - 4.0 µg/mL.
• Molecular Mass: Recombinant Mouse Sema5b, Fc Chimera has a calculated MW of 113.3 kDa (monomer). In SDS-PAGE migrates as 130-150 kDa, reducing conditions.
• Purity: >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).

Gene Information

• Gene Name: Sema5b sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B [ Mus musculus (house mouse) ]
• Official Symbol: Sema5b
• Synonyms: Sema5b; sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5B; SemG; Semag; AI893641; mKIAA1445; semaphorin-5B; sema G; semaphorin 5B; semaphorin-G
• Gene ID: 20357
• mRNA Refseq: NM_013661
• Protein Refseq: NP_038689
• UniProt ID: Q60519
• Chromosome Location: 16; 16 B3