Active Recombinant Mouse Serpina1a protein(Met1-Lys413), hFc-tagged

• Cat.No.: Serpina1a-472M
• Product Overview: Recombinant Mouse Serpina1a (XP_003945711.1) (Met1-Lys413) was expressed in HEK293 with the Fc region of Human IgG1 at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: Met1-Lys413
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substRate, Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys (DNP)-NH2. The IC50 value is < 1 nM.
• Molecular Mass: The recombinant mouse Serpina1a comprises 627 amino acids and has a predicted molecular mass of 70.2 kDa. The apparent molecular mass of the protein is approximately 67-77 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.