Active Recombinant Mouse Serpina1c Protein, His-tagged

• Cat.No.: Serpina1c-618M
• Product Overview: Recombinant Mouse Serpina1c (Accession # NP_033271) Glu25-Lys413, fused with a C-terminal 10-His tag, was produced in Mouse myeloma cell line, NS0-derived.

Specification

• Source: Mammalian cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: Glu25
• Form: Supplied as a 0.2 µm filtered solution in Sodium Acetate, NaCl and Glycerol.
• Bio-activity: Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2.The IC50 value is <10 nM, as measured under the described conditions.
• Molecular Mass: Recombinant Mouse Serpina1c, His-tagged has a calculated MW of 45 kDa. In SDS-PAGE migrates as 60 kDa, reducing conditions.
• Protein length: Glu25-Lys413
• Purity: >95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
• Storage: Avoid repeated freeze-thaw cycles. No activity loss was observed after storage at: In lyophilized state for 1 year (4ºC); After reconstitution under sterile conditions for 3 months (-70ºC).
• Reconstitution: Reconstitute at 100 µg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Gene Information

• Gene Name: Serpina1c serine (or cysteine) peptidase inhibitor, clade A, member 1C [ Mus musculus (house mouse) ]
• Official Symbol: Serpina1c
• Synonyms: Pi3; Pi6; Wap3; Skalp; Spi1-3; Spi1-6; alpha-1-antitrypsin 1-3; alpha-1-antitrypsin 1-3; alpha-1 protease inhibitor 3; alpha-1 protease inhibitor 6; alpha-1-antitrypsin 1-6; apha-1 protease inhibitor 3; pre-elafin; serine (or cysteine) proteinase inhibitor, clade A, member 1c; serine protease inhibitor 1-3; serine protease inhibitor 1-6; serine protease inhibitor A1c; serpin A1c; trappin-2
• Gene ID: 20702
• mRNA Refseq: NM_009245.2
• Protein Refseq: NP_033271.1
• UniProt ID: Q00896
• Chromosome Location: 12 E; 12 52.98 cM