Active Recombinant Mouse Shh Protein
Cat.No. : | Shh-5862M |
Product Overview : | Purified recombinant protein of Mouse sonic hedgehog (Shh) without tag was expressed in E. coli. |
- Specification
- Gene Information
- Related Products
Description : | The C-terminal part of the sonic hedgehog protein precursor displays an autoproteolysis and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein into two parts (ShhN and ShhC) followed by the covalent attachment of a cholesterol moiety to the C-terminal of the newly generated ShhN. Both activities occur in the reticulum endoplasmic. Once cleaved, ShhC is degraded in the endoplasmic reticulum. |
Source : | E. coli |
Species : | Mouse |
Bio-activity : | Determined by its ability to induce alkaline phosphatase production by C3H/10T1/2 (CCL-226) cells. The expected ED50 for this effect is 0.5-1.0μg/mL. |
Molecular Mass : | 20 kDa |
AA Sequence : | IVIGPGRGFGKRRHPKKLTPLAYKQ FIPNVAEKTLGASGRYEGKITRNSE RFKELTPNYNPDIIFKDEENTGADR LMTQRCKDKLNALAISVMNQWPGVK LRVTEGWDEDGHHSEESLHYEGRAV DITTSDRDRSKYGMLARLAVEAGFD WVYYESKAHIHCSVKAENSVAAKSG G |
Endotoxin : | < 0.1 ng/μg of protein (< 1 EU/μg) |
Purity : | > 90% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Unopened vial can be stored between 2 and 8 centigrade for up to 2 weeks, at -20 centigrade for up to 6 months, or at -70 centigrade or below until the expiration date. Aliquots can be stored between 2 and 8 centigrade for up to one week and stored at -20 centigrade or colder for up to 3 months. Avoid repeated freeze/thaw cycles. |
Storage : | Store at -80 centigrade. |
Storage Buffer : | LyopH ilized from a 0.2 μM filtered solution of 20 mM pH ospH ate buffer, 100 mM NaCl, pH 7.2 |
Gene Name : | Shh sonic hedgehog [ Mus musculus (house mouse) ] |
Official Symbol : | Shh |
Synonyms : | SHH; sonic hedgehog; sonic hedgehog protein; HHG-1; short digits; hemimelic extra toes; Hx; Dsh; Hhg1; Hxl3; M100081; 9530036O11Rik |
Gene ID : | 20423 |
mRNA Refseq : | NM_009170 |
Protein Refseq : | NP_033196 |
UniProt ID : | Q62226 |
Products Types
◆ Recombinant Protein | ||
SHH-7285H | Active Recombinant Human SHH protein(Met1-Gly197), His-tagged | +Inquiry |
SHH-852M | Recombinant Mouse SHH Protein (Ile24-Gly198), His-tagged | +Inquiry |
SHH-058S | Active Recombinant Human SHH Protein (176 aa) | +Inquiry |
Shh-310S | Active Recombinant Mouse Shh Protein (176 aa) | +Inquiry |
SHH-242R | Recombinant Rat SHH Protein | +Inquiry |
◆ Lysates | ||
SHH-1176MCL | Recombinant Mouse SHH cell lysate | +Inquiry |
SHH-1494HCL | Recombinant Human SHH cell lysate | +Inquiry |
SHH-1533HCL | Recombinant Human SHH cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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