Active Recombinant Mouse Tfrc protein, His-tagged, Low Endotoxin

• Cat.No.: TFRC-1290M
• Product Overview: Recombinant Mouse Tfrc protein(Q62351)(89-763 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 89-763 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS, 200mM L-arginine (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Mouse Transferrin R, His Tag at 5μg/ml(100μl/well) on the plate. Dose response curve for Biotinylated Human Transferrin, His Avi Tag with the EC50 of 14.5ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 76.99 kDa. Due to glycosylation, the protein migrates to 78-90 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 90% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Tfrc transferrin receptor [ Mus musculus ]
• Official Symbol: Tfrc
• Synonyms: TFRC; transferrin receptor; transferrin receptor protein 1; mammary tumor virus receptor 1; TR; TFR; p90; CD71; TFR1; Trfr; Mtvr1; Mtvr-1; AI195355; AI426448; AU015758; 2610028K12Rik; E430033M20Rik
• Gene ID: 22042
• mRNA Refseq: NM_011638
• Protein Refseq: NP_035768