Species : |
Mouse |
Source : |
HEK293 |
Tag : |
His |
Protein Length : |
57-235 aa |
Form : |
Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300. |
Bio-activity : |
TNF-a, being an endogenous pyrogen, is able to induce fever, necrosis, inflammation and to inhibit tumorigenesis. As reported, TNF-a could inhibit the proliferation and induce necrosis of A549 cells, and the concentration of IL-1β in cell supernatant will increase after stimulation. Therefore, A549 cells were incubated in DMEM with TNFa (1ng/mL, 10ng/mL) for 2h, 4h, 8h, 24h, 48h, then cells were observed by inverted microscope and IL-1β was detected in the cell supernatant by ELISA . |
Molecular Mass : |
21.4kDa |
Endotoxin : |
<1.0EU per 1μg (determined by the LAL method). |
Purity : |
> 95% as determined by SDS-PAGE. |
Storage : |
Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months. |
Reconstitution : |
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex. |