Active Recombinant Mouse TNF protein, His-tagged

• Cat.No.: TNF-0807M
• Product Overview: Recombinant Mouse TNF protein(Leu80-Leu235), fused with His tag, was expressed in HEK293.

Specification

• Source: HEK293
• Species: Mouse
• Tag: His
• Form: Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
• Bio-activity: Immobilized Mouse TNF-alpha, His Tag at 0.2 μg/mL (100 μL/well) can bind Human TNFR1, Fc Tag with a linear range of 0.1-1 ng/mL. Immobilized Mouse TNF-alpha, His Tag at 0.2 μg/mL (100 μL/well) can bind Humira with a linear range of 0.1-1 ng/mL.
• Molecular Mass: This protein carries a polyhistidine tag at the C-terminusThe protein has a calculated MW of 19.1 kDa. The protein migrates as 19-20 kDa and 23-24 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Proteinlength: Leu80-Leu235
• Endotoxin: Less than 1.0 EU per μg by the LAL method.
• Purity: >95% as determined by SDS-PAGE.
• Storage: Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Tnf tumor necrosis factor [ Mus musculus ]
• Official Symbol: TNF
• Synonyms: TNF; tumor necrosis factor; TNF-a; TNF alpha; cachectin; tumor necrosis factor alpha; tumor necrosis factor-alpha; tumor necrosis factor ligand superfamily member 2; DIF; Tnfa; TNFSF2; Tnfsf1a; TNFalpha; TNF-alpha; MGC151434
• Gene ID: 21926
• mRNA Refseq: NM_013693
• Protein Refseq: NP_038721