Active Recombinant Mouse Tnfrsf11b Protein, His tagged

• Cat.No.: Tnfrsf11b-04M
• Product Overview: Recombinant mouse Tnfrsf11B (22-401 aa), fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: Insect Cells
• Tag: His
• Protein Length: 22-401 aa
• Description: Enables heparan sulfate binding activity. Involved in negative regulation of osteoclast differentiation. Acts upstream of or within extracellular matrix organization; negative regulation of odontogenesis of dentin-containing tooth; and negative regulation of tumor necrosis factor-mediated signaling pathway. Located in extracellular matrix. Is expressed in several structures, including arterial system; bone; gut; hemolymphoid system gland; and placenta. Used to study osteoporosis. Human ortholog(s) of this gene implicated in Paget's disease of bone; Paget's disease of bone 5; and osteoarthritis. Orthologous to human TNFRSF11B (TNF receptor superfamily member 11b).
• Form: Liquid
• AASequence: ETLPPKYLHYDPETGHQLLCDKCAPGTYLKQHCTVRRKTLCVPCPDHSYTDSWHTSDECVYCSPVCKELQSVKQECNRTHNRVCECEEGRYLEIEFCLKHRSCPPGSGVVQAGTPERNTVCKKCPDGFFSGETSSKAPCIKHTNCSTFGLLLIQKGNATHDNVCSGNREATQKCGIDVTLCEEAFFRFAVPTKIIPNWLSVLVDSLPGTKVNAESVERIKRRHSSQEQTFQLLKLWKHQNRDQEMVKKIIQDIDLCESSVQRHLGHSNLTTEQLLALMESLPGKKISPEEIERTRKTCKSSEQLLKLLSLWRIKNGDQDTLKGLMYALKHLKTSHFPKTVTHSLRKTMRFLHSFTMYRLYQKLFLEMIGNQVQSVKISCL
• Molecular Mass: 44.4 kDa
• Bio-activity: Measured by its ability to inhibit cytotoxicity using Jurkat human acute T cell leukemia cells in the presence of 2 ng/mL of human TRAIL. The ED50 range ≤ 50 ng/mL.
• Endotoxin: < 1 EU/μg of protein determined by LAL method
• Purity: > 90% by SDS-PAGE
• Application: SDS-PAGE, Bioactivity
• Note: For research use only. This product is not intended or approved for human, diagnostics or veterinary use.
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Storage Buffer: Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
• Concentration: 0.25 mg/mL (determined by absorbance at 280nm)

Gene Information

• Gene Name: Tnfrsf11b tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) [ Mus musculus (house mouse) ]
• Official Symbol: Tnfrsf11b
• Synonyms: Tnfrsf11b; tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin); Opg; TR1; OCIF; tumor necrosis factor receptor superfamily member 11B; osteoclastogenesis inhibitory factor; osteoprotegerin
• Gene ID: 18383
• mRNA Refseq: NM_008764
• Protein Refseq: NP_032790
• UniProt ID: O08712