Active Recombinant Mouse Vcam1 protein, His-tagged

• Cat.No.: Vcam1-4012M
• Product Overview: Recombinant Mouse Vcam1 protein(NP_035823.3)(Met1-Glu698), fused with His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Glu698
• Form: Lyophilized from sterile PBS, pH 7.4Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Bio-activity: Measured by the ability of the immobilized protein to support adhesion of U937 human histiocytic lymphoma cells. When 5 x 10E4 cells/well are added to mouse VCAM1 coated plates (10 μg/ml with 100 μl/well), approximately 70%-80% cells will adhere after 1 hour at RT.
• Molecular Mass: The secreted recombinant mouse VCAM1 consists of 685 amino acids and has a predicted molecular mass of 75.8 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmVCAM1 is approximately 90-100 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 97 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Vcam1 vascular cell adhesion molecule 1 [ Mus musculus ]
• Official Symbol: Vcam1
• Synonyms: VCAM1; vascular cell adhesion molecule 1; vascular cell adhesion protein 1; V-CAM 1; CD106; Vcam-1
• Gene ID: 22329
• mRNA Refseq: NM_011693
• Protein Refseq: NP_035823