Active Recombinant Mouse Vtn protein, His-tagged

• Cat.No.: Vtn-4054M
• Product Overview: Recombinant Mouse Vtn protein(Asp20-Lys478), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: 20-478 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. When 5×10^4cells/well are added to Vitronectin coated plates (5 µg/mL with 100 µL/well), approximately >80% will adhere after 30 minutes.
• Molecular Mass: 53.59 kDa
• Endotoxin: < 1 EU/μg of the protein by LAL method.
• Purity: ≥ 85 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Vtn vitronectin [ Mus musculus ]
• Official Symbol: Vtn
• Synonyms: VTN; vitronectin; S-protein; serum-spreading factor; Vn; AI256434
• Gene ID: 22370
• mRNA Refseq: NM_011707
• Protein Refseq: NP_035837