Active Recombinant Mouse Vtn protein(Met1-Lys478), His-tagged

• Cat.No.: Vtn-4054M
• Product Overview: Recombinant Mouse vitronectin (NP_035837.1) (Met 1-Lys 478) was expressed in HEK293, with a polyhistidine tag at the C-terminus.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Met1-Lys478
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Bio-activity: Measured by the ability of the immobilized protein to support the adhesion of DU145 human prostate carcinoma cells. When cells are added to mouse Vitronectin coated plates (10 μg/mL and 100 μL/well), > 60% cells will adhere specifically after 30 minutes at 37 °C.
• Molecular Mass: The secreted recombinant mouse vitronectin consists of 470 amino acids and has a predicted molecular mass of 54.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rmvitronectin is approximately 75-85 kDa due to glycosylation.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 85 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Vtn vitronectin [ Mus musculus ]
• Official Symbol: Vtn
• Synonyms: VTN; vitronectin; S-protein; serum-spreading factor; Vn; AI256434
• Gene ID: 22370
• mRNA Refseq: NM_011707
• Protein Refseq: NP_035837