| Species : |
Mouse |
| Source : |
CHO |
| Protein Length : |
Wnt3: 22-355 aa
Sfrp1: 32-314 aa |
| Description : |
Wnt-3 is one of 19 vertebrate members of the Wingless-type MMTV integration site (Wnt) family of highly conserved cysteine-rich secreted glycoproteins that are important for normal developmental pr centigradeesses. Mouse Wnt-3 shares 99% and 100% amino acid (aa) identity with human and rat Wnt-3, respectively. It also shares 87% aa identity with mouse Wnt3a. Wnts bind to the cell surface Frizzled family receptors in conjunction with low-density lipoprotein receptor-related protein family receptors (LRP5 or 6) resulting in the stabilization of intracellular beta -catenin levels. As intracellular beta -catenin levels rise, it binds to TCF/LEF transcription factors, leading to expression of Wnt target genes. Wnts are known to bind to sFRPs, a family of secreted molecules that contain an N-terminal cysteine-rich domain (CRD) highly similar to the CRDs of the Frizzled family receptors. More than one molecule of sFRP is suggested to participate in the interaction with each Wnt molecule. Both sFRP-1 and sFRP-2 can inhibit Wnt-3a activity while sFRP-3 cannot. |
| Form : |
Non-covalent complex |
| Molecular Mass : |
37 kDa (Wnt-3) & 33 kDa (sFRP-1) |
| Bio-activity : |
Measured by its ability to activate Wnt induced TCF reporter activity in HEK293 human embryonic kidney cells. The ED50 for this effect is 7.00-63.0 ng/mL. |
| Endotoxin : |
< 0.10 EU/μg of the protein by the LAL method. |
| Purity : |
> 90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie Blue Staining. |
| N-terminal Sequence Analysis : |
Gly22 (Wnt-3) & Ser32 (sFRP-1) |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Storage Buffer : |
Lyophilized from a 0.2 μm filtered solution in MOPS and NaCl with Trehalose. |
| Reconstitution : |
Reconstitute at 100 μg/mL in PBS. |
| Shipping : |
The product is shipped at ambient temperature. |
