Active Recombinant Pig IL2 protein, His-tagged

• Cat.No.: IL2-1338P
• Product Overview: Active Recombinant Pig IL2 protein(P26891)(21~154 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Pig
• Source: HEK293
• Tag: His
• Protein Length: 21-154 aa
• Form: Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Bio-activity: IL-2 (Interleukin-2) is a cytokine produced by T-cells in response to antigenic or mitogenic stimulation. IL-2 is a type of signaling molecule in the immune system, that is required for both T-cell and B-cell proliferation and other activities crucial to regulation of the immune response.Therefore, in order to detect the bioactivity of recombinant pig IL-2, spleen single suspensions were prepared, activated with conA (final concentration 3 ug/ml). Cells were collected after 72h and washed with hanks.Then mouse splenic lymphocytes were were seeded into triplicate wells of 96-well plates at a density of 10,000 cells/well with or without the addition of various concentrations of recombinant pig IL-2. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). 10 µl of CCK-8 solution was added to each well of the plate, the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37°C. Proliferation of Splenic lymphocytes cells after incubation with IL-2 for 96h observed by inverted microscope was shown. The dose-effect curve of recombinant pig IL-2 was shown. It was obvious that recombinant pig IL-2 significantly promoted cell proliferation of Splenic lymphocytes cells. The EC50 for this effect is typically 0.0072-0.0079 ug/ml.
• Molecular Mass: 16.8kDa
• Endotoxin: <1.0EU per 1μg (determined by the LAL method).
• Purity: > 90% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: IL2 interleukin 2 [ Sus scrofa ]
• Official Symbol: IL2
• Synonyms: IL2; interleukin 2; interleukin-2; T-cell growth factor; IL-2; TCGF; POIL2
• Gene ID: 396868
• mRNA Refseq: NM_213861
• Protein Refseq: NP_999026